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KMID : 1098420050130050237
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 5 p.237 ~ p.241
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Production of Tropane Alkaloids by Optimal Culture Conditions in Adventitious Root of Hyoscyamus niger L.
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Park Dong-Jin
Min Ji-Yun
Kim Yong-Duck
Kang Seung-Mi
Jung Ha-Na
Kang Hoon-Serg
Choi Myung-Suk
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Abstract
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Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and roots were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best hyoscyamine productivity was resulted in MS medium. Sucrose was increased scopolamine and hyoscyamine production were increased the medium supplemented by sucrose comparing to than those by other carbon sources.
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KEYWORD
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Hyoscyamus niger L., adventitious roots, scopolamine, hyoscyamine
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